Citation
The expression and influence of IFN-y in Zip14 ablated mice

Material Information

Title:
The expression and influence of IFN-y in Zip14 ablated mice
Creator:
Davis, Ethan John Allen
Publisher:
University of Florida
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
Undergraduate Honors Thesis/Project

Notes

Abstract:
Zip14 (Slc39a14) is a zinc transporter involved in the anti-inflammatory properties of this mineral. It can be found in the basolateral membrane of intestinal epithelial cells (IECs) especially when exposed to proinflammatory stimuli. Deletion of Slc39a14, and subsequent lack of the Zip14 transporter, has been shown to induce a dysregulated gut environment conducive to inflammation. RNA sequencing was used to profile the proteins upregulated in inflammatory conditions. In past research, IFN-?—an immunomodulatory cytokine that plays a crucial role in both innate and adaptive immune responses—has been shown to upregulate when the gut environment deviates from homeostasis. Using a newly available murine intestinal cell culture line, this paper examines RNA expression of inflammatory mediators between three groups: Zip14 ablation with IFN-?, Zip14 ablation, and Zip14 intact. Utilizing qPCR, we show that Zip14 ablation has a trend toward upregulation of IFN-?, IL-6, and STAT3. In addition, IECs may be a source of IFN-? in intestinal inflammation. In the presence of IFN-?, we conclude that further upregulation of IL-6 and STAT3 occurs. ( en )
General Note:
Awarded Bachelor of Science, magna cum laude, on May 6, 2023. Major: Nutritional Sciences
General Note:
College or School: Agricultural and Life Sciences
General Note:
Advisor: Robert J. Cousins. Advisor Department or School: Food Science and Human Nutrition.

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Source Institution:
University of Florida
Holding Location:
University of Florida
Rights Management:
Copyright Ethan John Allen Davis. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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PAGE 18

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PAGE 19

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PAGE 20

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PAGE 21

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PAGE 22

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!-- The expression and influence of IFN y in Zip14 ablated mice ( Book ) --
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mods:abstract lang en Zip14 (Slc39a14) is a zinc transporter involved in the anti-inflammatory properties of this mineral. It can be found in the basolateral membrane of intestinal epithelial cells (IECs) especially when exposed to proinflammatory stimuli. Deletion of Slc39a14, and subsequent lack of the Zip14 transporter, has been shown to induce a dysregulated gut environment conducive to inflammation. RNA sequencing was used to profile the proteins upregulated in inflammatory conditions. In past research, IFN-?—an immunomodulatory cytokine that plays a crucial role in both innate and adaptive immune responses—has been shown to upregulate when the gut environment deviates from homeostasis. Using a newly available murine intestinal cell culture line, this paper examines RNA expression of inflammatory mediators between three groups: Zip14 ablation with IFN-?, Zip14 ablation, and Zip14 intact. Utilizing qPCR, we show that Zip14 ablation has a trend toward upregulation of IFN-?, IL-6, and STAT3. In addition, IECs may be a source of IFN-? in intestinal inflammation. In the presence of IFN-?, we conclude that further upregulation of IL-6 and STAT3 occurs.
mods:accessCondition Copyright Ethan John Allen Davis. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
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mods:note Awarded Bachelor of Science, magna cum laude, on April 29, 2022. Major: Nutritional Sciences
College or School: Agricultural and Life Sciences
Advisor: Robert J. Cousins. Advisor Department or School: Food Science and Human Nutrition. Advisor: . Advisor Department or School:
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mods:title The expression and influence of IFN-y in Zip14 ablated mice
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