Citation
Proliferating cell nuclear antigen as a marker for investigating gonadal function in male Florida manatees (Trichechus manatus latirostris)

Material Information

Title:
Proliferating cell nuclear antigen as a marker for investigating gonadal function in male Florida manatees (Trichechus manatus latirostris)
Series Title:
18th Annual Undergraduate Research Symposium
Creator:
Quiñones, Maria
Language:
English

Subjects

Subjects / Keywords:
Center for Undergraduate Research
Biological Sciences
Genre:
Conference papers and proceedings
poster ( aat )

Notes

Abstract:
Investigating gonadal function can provide insight into the reproductive potential of individual males of a particular species. For the protected Florida manatee (Trichechus manatus latirostris), this represents a crucial step towards gaining a better understanding of reproduction, which is necessary to provide better information for management and conservation efforts. The aim of this study was to investigate gonadal function by assessing spermatogonial germ cell proliferation within gonadal tissues collected from male Florida manatees. We hypothesized that spermatogonial germ cell proliferation would be highest in adult manatees. Gonadal tissues were collected from salvaged carcasses brought into the Florida Fish and Wildlife Pathobiology Laboratory within 24 – 48 hours post-mortem. Tissue sections were immunostained with a rabbit monoclonal anti-proliferating cell nuclear antigen (PCNA) primary antibody at a 1:500 dilution. Positive nuclear staining of spermatogonial germ cells was present in all immunostained tissue sections. The seminiferous tubules within each tissue section displayed a large amount of heterogeneity in proliferation amongst all samples. PCNA expression within the seminiferous epithelium presented clear qualitative stepwise differences between the developmental age-classes. The highest levels of expression were present in the adult males followed by moderate levels in the juveniles and the lowest levels in the calves. ( en )
General Note:
Research Authors: Maria I. Quinones, Jonathan R. Cowart, Iskande L. V. Larkin - University of Florida
General Note:
University Scholars Program
General Note:
Faculty Mentor: Iske Larkin - LACS, University of Florida

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Source Institution:
University of Florida
Rights Management:
Copyright Maria Quiñones. Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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