- Permanent Link:
- https://ufdc.ufl.edu/AA00054860/00038
Material Information
- Title:
- Stretching Device for Mechanical Cellular Stimulation
- Series Title:
- 18th Annual Undergraduate Research Symposium
- Creator:
- Kurosu Jalil, Myra
- Language:
- English
Subjects
- Subjects / Keywords:
- Center for Undergraduate Research
Engineering
- Genre:
- Conference papers and proceedings
poster ( aat )
Notes
- Abstract:
- Cells respond to mechanical changes such as applied strain in their micro-environments. We are interested in studying how Acomys, or spiny mouse, cells respond to these mechanical stimuli. The spiny mouse is unique in that its skin does not scar while healing, and we hypothesize that its response to applied strain is part of Acomys’ unique, non-scarring behavior. We designed A Live Biomimetic Real-Time Actuator (ALBRTA) to fit under the microscope and stretch cells. ALBRTA is a mini tensile tester designed to accommodate soft materials such as flexible silicone constructs. Constructs with cells seeded on them are clamped down at each end and pulled apart using two high-precision stepper motors to apply tension. Screws are used to convert the motors’ rotational motion into linear motion. The number and direction of turns (which determine the strain) are programmed using an Arduino microcontroller. Multiple manual iterations of ALBRTA were manufactured and tested before it was automated. ALBRTA has been calibrated so that a specific number of turns of the motor results in a known strain value of the silicone constructs. The device is now being used to stretch and observe normal mouse cells and later will be used on Acomys cells. ( en )
- General Note:
- Research Authors: Chelsey Simmons, Justin Keister, Myra Kurosu Jalil - University of Florida
- General Note:
- Faculty Mentor: Chelsey Simmons - Department of Mechanical and Aerospace Engineering, University of Florida
Record Information
- Source Institution:
- University of Florida
- Rights Management:
- Copyright Myra Kurosu Jalil. Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
|
PAGE 1
Stretching Device for Mechanical Cellular Stimulation Myra Kurosu Jalil, Justin Keister, Dr. Chelsey Simmons Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL Design References Introduction Cells respond to mechanical changes such as applied strain in their microenvironments. The skin of the spiny mouse does not scar while healing. We hypothesize that its response to applied strain is part of Acomys unique, non -scarring behavior. We built a stretching device to study the response of Acomys cells to mechanical stimuli. We also designed silicone constructs to seed the cells on. Calibration Results Fibroblast cells from normal mice can be plated, stretched, and imaged in real time. 1. A. J. Engler S. Sen, H. L. Sweeney, and D. E. Discher Matrix Elasticity Directs Stem Cell Lineage Specification, Cell vol. 126, no. 4, pp. 645647, Aug. 2006. 2. W W. Ahmed, M. H. Kural and T. A. Saif A novel platform for in situ investigation of cells and tissues under mechanical strain, Acta Biomaterialia vol. 6, no. 8, pp. 29792990, Feb. 2010. 3. N Calvo Applications of dynamic cell culture systems using stretchable silicone constructs for in vitro models and methods, M.S. thesis, Dept. Mech. Eng., Univ. Florida, Gainesville, 2016 Future Work The device is now being used to stretch and observe normal mouse cells and will later be used to stretch and observe Acomys cells. The stepper motors initially used were found to have inadequate torque ratings. They will be replaced with new stepper motors that can withstand higher torque and will be able to generate constant strain. Calibration will be repeated with the new motors. We expect better values after the second round. A Live Biomimetic Real -Time Actuator ( ALBRTA ) that fits under the microscope and stretches cells. ALBRTA is a mini tensile tester designed to accommodate soft materials such as flexible silicone constructs. Constructs with cells seeded on them are clamped down at each end and pulled apart using two high -precision stepper motors to apply tension. Due to the geometry of the constructs, the resulting strain is transferred to the cells. Screws are used to convert the motors rotational motion into linear motion. The number and direction of turns are programmed using an Arduino microcontroller Multiple manual iterations of ALBRTA were manufactured and tested before it was automated. ALBRTA has been calibrated so that a specific number of turns of the motor results in a known strain value of the silicone constructs. ALBRTA can be set up under a microscope to perform stretching experiments. 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 0 1/2 1 1 1/2 2 2 1/2Average Translation per Rotation (mm) Rotations Translation/Rotation vs. Rotations Average Translation/CCW Rotation (mm) Average Translation/CW Rotation (mm) Ideal Translation/RotationAcknowledgements The author would like to thank Dr. Simmons, Justin Keister and all members of GatorBAIT laboratory for their guidance and support. ALBRTA connects to the Arduino board, which powers and sends signals to the motors. A CAD model of the latest version of ALBRTA. The frame can be easily disassembled to facilitate the insertion of the constructs. ALBRTA was calibrated by programming the motors to turn a specified number of steps. We watched how far the stages travelled under a microscope and took pictures after each run. An analysis of the images yielded the values below:
|